Nature research paper: A discrete neuronal population coordinates brain-wide developmental activity
. MIPs of half-brain confocal stacks from different times during pupal development and early adult life. Nuclei of mCherry-NLS expressing Trpγ+ neurons shown ; reference marker is Ncad . Average, SD, and number of samples for each time point are printed top-right of panels; these values are plotted in Fig..13µm-thick MIP of a 72 hAPF brain stained for neuronal nuclei , Trpγ+ nuclei , and a reference marker . Image derived from three stitched confocal stacks. Scale bar, 100µm.
13µm-thick MIP of a 72 hAPF brain stained for glial nuclei , Trpγ+ nuclei , and a reference marker . Image derived from three stitched confocal stacks. Scale bar, 100µm.Histogram of average voxel intensities of segmented Repo+ and Trpγ+ nuclei measured in the anti-Repo channel of the top image. n=5055 , 1464 ; half-brain complements analyzed. Inset shows where 9/1464 Trpγ+ cell intensities overlap with the dimmest Repo+ glia.
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