Biocatalytic synthesis of pseudouridine for cheaper mRNA vaccines NatureComms
About 1–2 μL sample were spotted onto a TLC plate . The mobile phase was composed of 2- BuOH:AcOH:H. Spots were developed by heating the plate to ~70 °C for 2 min using a heat gun.2
O in a total volume of 600 μL and transferred to a 5 mm high-precision NMR sample tube. Samples were analyzed on a Varian INOVA 500-MHz NMR spectrometer using the VNMRJ 2.2D software for the measurements, or on a Bruker AVANCE III 300-MHz spectrometer with an autosampler and the Bruker Topspin 3.5 software for measurements.The standard conditions on which to perform single-parameter variation were 1.5 M potassium phosphate buffer , 1.0 M, 1.0 mg/mL UP, 10 mg/mL DeoB and 6.0 mg/mL YeiN.
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