Nature research paper: Ancient human DNA recovered from a Palaeolithic pendant
As sodium phosphate buffer did not cause substantial alterations of ancient bones and teeth in 3DST measurements, we modified a previously described method for temperature-controlled gradual DNA release from bone and tooth powder using this reagentfor non-destructive DNA extraction from complete bones and teeth.
Negative controls containing sodium phosphate buffer or lysis buffer without sample material were carried alongside the samples through all steps of subsequent sample preparation and sequencing. For a subset of samples, DNA was extracted from only the first phosphate buffer fraction produced at each of the four incubation temperatures. Supplementary DataOf the extract, 10 µl was then converted into single-stranded DNA libraries using the automated protocol described in Gansauge et al.
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