Nature research paper: Brain-restricted mTOR inhibition with binary pharmacology
streptomycin . All cell lines tested mycoplasma negative using the MycoAlert Mycoplasma Detection Kit . Cell lines from the ATCC were short randem repeat profiled by the manufacturer. Jurkat-Lucia NFAT cells are a commercial cell line developed by InvivoGen and was authenticated by the manufacturer. Cell lines from UCSF Cell Culture Facility were STR profiled by the UCSF Cell Culture Facility. No further authentications were performed after the purchase.
The TR-FRET signal was calculated as the ratio fluorescence intensity [read B]/[read A]. Data were plotted in GraphPad Prism 9.0.HEK293T cells were transfected with standard packaging vectors using the TransIT-LT1 Transfection Reagent . Viral supernatant was collected 2–3 days after transfection, filtered through 0.45-μm polyvinylidene difluoride filters and frozen at –80 °C before transduction.were cloned into pCRISPRia-v2 .
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