Nature research paper: Brown-fat-mediated tumour suppression by cold-altered global metabolism
values and migration times of corresponding authentic standard compounds. Data were quantified by comparing the ratio of analyte peak area/internal standard peak area of sample solution to that of the reference standard mixture. The quantitative formula was as follows:= / × × ) × 1/ × )/). The sample relative area = /. The standard relative area = /. Chromatograms of each metabolite were extracted and quantified using the Agilent MassHunter Qualitative Analysis software .
Mice in thermoneutral and cold environments were anaesthetized and kept at room temperature for 5 min for imaging. Thermal images on the back of mice were captured using an infrared thermal imager . Setting environmental temperature as the baseline, thermal images of mice were acquired and were further analysed using Fotric AnalyzIR.The paraffin-embedded tissue sections of 5 μm thickness were incubated at 60 °C for approximately 2 h to melt the covered paraffin.
Total RNA was extracted from cells and tissues using the TRIzol and GeneJET RNA Purification Kits according to the manufacturer’s instructions. The total RNA was reverse-transcribed and cDNAs were used for PCR and qPCR analyses using the primers listed in Supplementary Table. Samples were stored at −20 °C and processed for qPCR using the ABI Prism 7500 System .
For patients with cancer, an 18-year-old female patient with Hodgkin’s lymphoma participated in a pilot study during the intervals of the fifth cycle of combination chemotherapy of adriamycin, bleomycin, vinblastine and dacarbazine for 5 cycles. The cold ambient temperature was approximately 22 °C regulated by air-conditioning for 1 week and the patient wore light clothing. The patient fasted overnight before PET–CT examination.
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