A marine mystery: Finding the link between climatechange and sea sponge loss
]. Analysis of variance was used to test the effect of pH, temperature and the interaction between the two factors on host health using the R aov function. For the microbiome composition, since a relatively low number of taxa was identified , they were all retained to assess the impact of OW, OA and the combination of factors.
To further identify the biological processes impacted by OW, KEGG modules were identified within the dataset using the “annotate” and “classify” functions of enrichM . Only modules that were at least 70% complete were kept for further analysis. KOs identified across all metagenomes fell into 219 prokaryotic modwere kept for statistical analyses by EdgeR, which was run with the same settings as for the KOs analysis.
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