Nature research paper: Left–right symmetry of zebrafish embryos requires somite surface tension
Raw zoomed in image of a representative region of an embryo imaged using Viventis microscope. Scale bar, 25 μm. Image in overlaid with segmented notochord and somite boundaries . Segment length is determined with respect to the notochord. Yellow arrow represents local angle of the notochord. Scale bar, 25 μm. , Quiver plots of the eigen vectors upon application of Frangi vesselness filter. Orientation of the vectors represent directions of least change in fluorescent intensity.
, Lines that are less than 15 pixels apart are joined together by straight lines following which lines that do not represent notochord or somite boundaries are discarded. , Any remaining gaps in boundary detection are then connected by straight lines. Further details on statistics and reproducibility can be found in the Methods section. Initial AP lengths, lengths at 1 hr and lengths at 2 hr of somites one to eight are shown across the six multiview datasets.
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