In an open-label, randomized controlled trial, normothermic machine perfusion of kidneys from donation after circulatory death was found to be feasible and safe but did not reduce the rate of delayed graft function compared to static cold storage.
Eligible patients enrolled on the transplant waiting list and allocated a suitably matched kidney were enrolled at four UK transplant centers. Inclusion criteria included recipients 18 years of age or older with end-stage renal failure requiring their first or second kidney transplant who received a kidney from Maastricht category III or IV DCD donors 18 years of age or older.
. After the 1-h period of NMP, kidneys were flushed with hyperosmolar citrate solution at 4 °C to remove the red-cell-based perfusate and to re-cool the organ before transplantation. NMP kidneys were stored in ice and transplanted as soon as possible.Kidneys were transplanted into either iliac fossa with anastomosis of the artery to the common, external or internal iliac arteries. The vein was anastomosed to either the common or the external iliac vein.
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