Researchers used structured light and switchable fluorescent molecules to reduce the background light from the out-of-plane regions of microscope samples. This method allowed for the acquisition of images that surpassed the conventional resolution limit, and it may be useful for further study of cell clusters and other biological systems.
Researchers at Osaka University employ fluorescent molecules that can switch on and off, along with selective illumination in a plane, to create sharper microscope images that can be used to visualize internal cell structures or cell clusters.
While some previous methods utilized random fluorescence emission from single molecules, or"donut" shaped second light source to deactivate or deplete fluorescent sources outside of a desired area, this new method can be gentler on cells that might be damaged by intense or long exposure to light.
Biology Biotechnology Developmental Biology Optics Graphene Biochemistry Organic Chemistry
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