The overlooked variable in animal studies: why diet makes a difference

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The overlooked variable in animal studies: why diet makes a difference
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Careful consideration and documentation of laboratory animals’ diets will boost the reproducibility of experiments

. Scientists then went on to develop multiple standardized diets for animal facilities and laboratories, leading to the production of various standardized pellet foods for laboratory rats and mice. “It became very clear that nutrition was going to be one of the key components in improving rigour and reproducibility,” Watts says.

Even in short-term studies — and even with standardized diets — experimental complications crop up. In 2018, dietitian Caroline Tuck at La Trobe University in Melbourne, Australia, was studying short-chain fermentable carbohydrates known as FODMAPs, which can exacerbate conditions such as irritable bowel syndrome in humans.

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