Visualizing translation dynamics at atomic detail inside a bacterial cell - Nature

Norge Nyheter Nyheter

Visualizing translation dynamics at atomic detail inside a bacterial cell - Nature
Norge Siste Nytt,Norge Overskrifter
  • 📰 physorg_com
  • ⏱ Reading Time:
  • 47 sec. here
  • 2 min. at publisher
  • 📊 Quality Score:
  • News: 22%
  • Publisher: 55%

Seeing antibiotics in action inside a pathogenic bacterium nature

To further refine the ribosome–ribosome interface in polysomes, RELION classification was performed with sub-tomograms extracted with a large box size that can accommodate two ribosomes. After refinement on the preceding ribosome , classification without refinement was performed with a local mask focusing on the following ribosome . Only ribosome pairs within tightly packed polysomes resulted in average densities with both ribosomes well resolved.

Permutation analysis was performed to test the significance of differences between occurrence frequencies of the experimental pairs compared to the theoretical pairs. The ribosome pair sequences in polysomes were represented as a matrix with single polysomes as rows and ribosome positions as columns, in which each matrix cell contains the ribosome class representing its elongation state. Columns with positions beyond each ribosome’s length were assigned to NaN .

For polysome distance threshold analysis, matrices were created from the full ribosome dataset by varying the distance threshold to the nearest neighbour in the range from 3 to 10 nm. The distances for polysome definition were calculated between the mRNA exit site of one ribosome and the mRNA entry site of another ribosome as described above. The fractions of elongation states in ribosome pairs were calculated as for the original ribosome set .

Vi har oppsummert denne nyheten slik at du kan lese den raskt. Er du interessert i nyhetene kan du lese hele teksten her. Les mer:

physorg_com /  🏆 388. in US

Norge Siste Nytt, Norge Overskrifter

Similar News:Du kan også lese nyheter som ligner på denne som vi har samlet inn fra andre nyhetskilder.

Arming T cells against B cells in systemic lupus erythematosus - Nature MedicineArming T cells against B cells in systemic lupus erythematosus - Nature MedicineCARTtherapy induced remission in 5 patients with systemic lupus erythematosus. In our latest News and Views, Jane Salmon WeillCornell puts the data in context and discusses next steps for the field. SLE lupus CARTcells
Les mer »

Haplotype-aware analysis of somatic copy number variations from single -cell transcriptomes - Nature BiotechnologyHaplotype-aware analysis of somatic copy number variations from single -cell transcriptomes - Nature BiotechnologyNumbat integrates expression, allele and haplotype information to detect copy number variations in scRNA-seq.
Les mer »

CAR T cells with dual targeting of CD19 and CD22 in pediatric and young adult patients with relapsed or refractory B cell acute lymphoblastic leukemia: a phase 1 trial - Nature MedicineCAR T cells with dual targeting of CD19 and CD22 in pediatric and young adult patients with relapsed or refractory B cell acute lymphoblastic leukemia: a phase 1 trial - Nature MedicineBicistronic CART cells targeting CD19 & CD22 show clinical activity in pediatric & young adult patients w/ B cell acute lymphoblastic leukemia, w/ relapses associated w/ limited CAR T cell persistence. ChildhoodCancerAwarenessMonth NMEDclinical
Les mer »

Oakland A’s open final road trip of season with 4-3 loss to AngelsOakland A’s open final road trip of season with 4-3 loss to AngelsA’s lose game No. 98, and now have eight remaining to avoid the second 100-loss season in Oakland franchise history.
Les mer »

MLB power rankings: Who's going to lose 100 games? Several teams hope to avoid ugly milestoneMLB power rankings: Who's going to lose 100 games? Several teams hope to avoid ugly milestoneJust 1.5 games separate the New York Mets from the Atlanta Braves in the NL East race, despite both teams clinching a playoff spot already. With an eye toward the postseason, here are the MLB power rankings via JesseYomtov
Les mer »

‘Something is seriously wrong’: Room-temperature superconductivity study retractedAfter doubts grew, a blockbuster paper claiming to have discovered a room-temperature superconductor has been withdrawn by Nature over objections of the study team.
Les mer »



Render Time: 2025-03-15 09:57:09