Nature research paper: Phytocytokine signalling reopens stomata in plant immunity and water loss
, Predicted structures of SCREW1 and SCREW2 C-terminal 23 aa. Structures were predicted using AlphaFold Protein Structure Database . Disulfide bonds are shown by yellow sticks., Two cysteine residues are required for SCREW2 activation of
s. Ten-day-old plate-grown WT seedlings were treated with or without 100 nM SCREW2, SCREW2, SCREW2 and SCREW2. activation was analysed by immunoblots with anti-pERK1/2 antibodies , and the protein loading is shown by CBB staining for RBC ., Two cysteine residues are required for SCREW2-induced seedling growth inhibition. Three-day-old plate-grown WT seedlings were transferred into liquid ½MS medium without or with 1 μM peptides. Images were taken , and fresh weights of seedlings were measured seven days later.
activation. Ten-day-old plate-grown WT seedlings were treated with or without 100 nM SCREW1, biotin-SCREW1, and SCREW1-HA. activation was analysed by immunoblots using anti-pERK1/2 antibodies with the protein loading shown by CBB staining for RBC ., The biotin-SCREW1 and SCREW1-HA peptides have similar activities with SCREW1 for seedling growth inhibition. The experiment was performed as in . Data are shown as box plots with the interquartile range as the upper and lower confines, minima and maxima as whiskers, and the median as a solid line . Experiments were repeated three times with similar results.
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